How much would it cost to build a CRISPR/Cas9 system using macrophages cell line? What machines and raw materials are needed?

How long it will take for a successful editing?

31 December 2018 5,741 1 View

Which breast cell line makes baso-lateral membrane,tight junctions and has high polarity?

To know polarity defect (by knocking out some specific genes) I am looking for a breast cancer cell line that has high polarity, forms tight junctions and easy to transfect.

25 July 2018 9,592 3 View

How much chlorine is present in 5.75% NaOCl. How to calculate it?

I am anlyzing bleaching powder sample (5.75%) through titration which gives the amount of chlorine present. How to convert chlorine content into NaOCl?

11 December 2017 1,566 0 View

While I was using WB, I found lots of scattered band where I never expected. What is the reason?

01 February 2016 3,249 4 View

Why did my cells disconnected during the immunofluorecence procedure?

I am trying to do IF to U2OS cell line in 96 wells plate. during the procedure I lose many cells which dissociate off the plate. Does anyone has a tip how to stay with many cells at the end of the...

28 June 2015 6,856 4 View

Which is an appropriate solvent for melatonin?

04 May 2015 1,869 14 View

Which solvent can I use to dissolve melatonin for the macrophage cell line?

I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its...

04 February 2015 9,889 3 View

Which solvent can I use to dissolve melatonin for the macrophage cell line?

I want to work with the macrophage cell line with melatonin. Which solvent and how can I use it for dissolving melatonin minimizing solvents adverse effects? Can you answer elaborately with its...

01 February 2015 2,685 4 View

How can I detect or measure several proteins or mRNA by a single method simultaneously?

I would like to measure the expression of several proteins or mRNA simultaneously. I mean, can I measure by one nitrocellulose membrane (as all of my target proteins or mRNA will be present there)...

01 February 2015 9,842 6 View

I am testing a great number of recently arrived refugees/ immigrants for polio seroprotection: what is the best method and what the levels of seroprotection?

Immunity

22 October 2012 2,197 1 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

What are anisotropic electrical and optical properties of a semiconductor due to?

In some materials, properties such as band gap, thermal conductivity differ along different crystallographic axes. What are the basics?

01 March 2021 1,923 3 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View