How much volume of primers will be added in a 25 µl reaction volume to make a final conc of 15 pmol from a primer stock solution of 20 µM?

More Beatrice Chipwaza's questions See All

Quick way to collect saliva to study viral RNA expression?

Hello, I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and...

24 September 2020 4,937 4 View

What have been the effects of Covid-19 on digital engagement in the Heritage industry?

For my dissertation i am looking at digital history and digital engagement and how this has been effected by the Covid-19 pandemic. Any help with this research would be greatly appreciated.

29 July 2020 2,808 3 View

Why my PCR amplicons no longer have the expected size and are faint?

Dear all, I have issues with my PCR amplicons. For one week now, I do no longer have the expected size for my PCR amplicon (252 bp in the first 4 wells) on the 50 bp DNA ladder. They appear faint...

17 July 2020 4,988 5 View

How can I make a comparison between LAMMPS results and lab results?

Dear all, I'm simulating the CO2 adsorption on two graphite sheets using the LAMMPS software and I calculated the density profiles in the pore width. Now I would like to compare the results with...

20 January 2020 4,211 1 View

Any NK cell line that express inhibitory killer cell immunoglobulins-like receptors (iKIRs)?

I wish to investigate the influence of iKIRS in natural killer cells cytotoxicity to the tumor cells, yet i dont seem to get any NK cell line that expresses iKIRS.

21 May 2018 3,100 1 View

Why my ligation presents non specific bands?

I'm tring to insert a fragment with 342pb in the pENTR/SD/D TOPO cloning vector, that have about 346pb. I have already purified the gel with the DNA fragment and did the ligation, using the...

17 January 2018 9,882 4 View

I want to preview BRATs medical image using MATLAB

I used the code "Read 3D data " in Matlab to read in my medical image but i cant view the image yet i guess it has to do with rescaling. this is the message in the command window. RescaleSlope not...

01 January 1970 6,083 5 View

Police leaving the force to join private investigations

Hi Everyone, I am looking for officers who have quit the force and joined the PI field for what ever reason. Last year it was reported that there was a shortage of officers because many quit the...

01 January 1970 1,813 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

Abhilash Deo

You have to take 0.75 microlitre of the 20 micromolar working stock. This corresponds to 15pmol.

Beatrice Chipwaza

Thank you Deo. Please, I will appreciate if you show me how did you obtain 0.75 microliter?

Katie A Burnette

C1V1 = C2V2

Concentration of stock * volume of stock = concentration of final * final volume

Just fill in your known values and solve for the volume of stock to add to your reaction.