How much volume of primers will be added in a 25 µl reaction volume to make a final conc of 15 pmol from a primer stock solution of 20 µM?

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Abhilash Deo

You have to take 0.75 microlitre of the 20 micromolar working stock. This corresponds to 15pmol.

Beatrice Chipwaza

Thank you Deo. Please, I will appreciate if you show me how did you obtain 0.75 microliter?

Katie A Burnette

C1V1 = C2V2

Concentration of stock * volume of stock = concentration of final * final volume

Just fill in your known values and solve for the volume of stock to add to your reaction.