Quick way to collect saliva to study viral RNA expression?

Hello, I am going to perform some saliva's RNA analysis. I found in the litterature several protocols to collect and prepare saliva for RNA/DNA analysis. For main of them we have to use kits and...

24 September 2020 4,937 4 View

What have been the effects of Covid-19 on digital engagement in the Heritage industry?

For my dissertation i am looking at digital history and digital engagement and how this has been effected by the Covid-19 pandemic. Any help with this research would be greatly appreciated.

29 July 2020 2,808 3 View

Why my PCR amplicons no longer have the expected size and are faint?

Dear all, I have issues with my PCR amplicons. For one week now, I do no longer have the expected size for my PCR amplicon (252 bp in the first 4 wells) on the 50 bp DNA ladder. They appear faint...

17 July 2020 4,988 5 View

How can I make a comparison between LAMMPS results and lab results?

Dear all, I'm simulating the CO2 adsorption on two graphite sheets using the LAMMPS software and I calculated the density profiles in the pore width. Now I would like to compare the results with...

20 January 2020 4,211 1 View

What is best method for Extraction Biofilm from Microsporum canis ?

Extraction Biofilm of Microsporum canis.

28 December 2019 776 4 View

Can DC motor be directly connected to solar panel, without using any battery??

I wish to connect a 500w 24V DC motor to solar panels, without using any battery. The rated current of motor is 27.6A. I have two solar panels with 24V and 325 watt ratings.The 'maximum power...

05 October 2019 5,086 6 View

Any NK cell line that express inhibitory killer cell immunoglobulins-like receptors (iKIRs)?

I wish to investigate the influence of iKIRS in natural killer cells cytotoxicity to the tumor cells, yet i dont seem to get any NK cell line that expresses iKIRS.

21 May 2018 3,100 1 View

Can anybody tell me the relevant references for design of buttress retaining wall?

Can anybody tell me the relevant references (book, Journal) for design of buttress retaining wall? Please tell if any

25 March 2018 6,736 1 View

Why my ligation presents non specific bands?

I'm tring to insert a fragment with 342pb in the pENTR/SD/D TOPO cloning vector, that have about 346pb. I have already purified the gel with the DNA fragment and did the ligation, using the...

17 January 2018 9,882 4 View

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19 April 2017 4,073 3 View

Glass fiber content in a gypsum board ?

Any idea on how to get the volumic ratio of glass fiber in a gypsum board ? Thank you for your help !

01 March 2021 2,501 3 View

How can I represent SDS-PAGE results for machine learning analysis?

I am trying to classify and analyze the results of an SDS-PAGE based array for bacterial detection using machine learning, but I have trouble finding the best way to represent the results with...

27 February 2021 9,176 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How can we limit artifacts caused by rotation in fiber photometry?

We are trying to run fiber photometry experiments for long time scales, but we get these sinusoidal artifacts that come up. We think they may be caused by twisting of the fiber optic subject...

25 February 2021 568 1 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Alginate and silk fibroin hydrogels toxic to cells?

Hello, I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to...

23 February 2021 8,249 2 View

How to properly calculate Interfacial area density in a porous domain?which are possible value for glass fiber?

I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...

23 February 2021 6,043 1 View

Diltuions for a Western blot?

I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...

22 February 2021 9,901 3 View