How much protein yield is expected from 50 murine islets (assuming average 2 K cells per islet)?

More Jay Patankar's questions See All

How to calculate corrosion rate from polarization curve when Cathodic protection is applied?

I believe its 10th such paper which just briefly mentioned how they used anodic current density at cathodic protection potential extracted from the polarization curves, to estimate the corrosion...

23 April 2024 1,541 3 View

Is it wise to add phosphatase inhibitors to the PFA while doing transcardiac perfusion in rats for detection of phosphoproteins using IHC?

I was trying to visualize phospho-ERK and phospho-Cofilin in rat brain sections. However, the images were disappointing and after doing some literature search, I realized that this may be due to...

07 April 2024 3,938 0 View

What could be the best method for multi-criteria decision making analysis for career preferences?

I have tried using the Analytical Hierarchical Process (AHP) for MCDM to evaluate the career preferences of graduates. However, I would like to know more about other best options.

13 March 2024 5,470 2 View

Selection dynamic model?

I have a dataset with T=11(2010-2020) and N=63(provinces). I want to estimate the causality relationship in short run and long run between 2 variables. Which model can be suitable?

10 March 2024 1,796 2 View

Why is there a need to teach Filipino Philosophy as Introduction to the Philosophy of the Human Person in Senior High School?

Filipino Philosophy is a mere course in Higher Education. I think that it is better to teach Filipino Philosophy as a core subject at the Senior High School Level.

25 February 2024 924 3 View

Which of the many modern theories of suicide do you find the most compelling, and why?

By this, I mean, one specific theory, whether widely known and accepted, almost as "conventional wisdom", or a peripheral theory few may be familiar with. What makes this theory particularly...

21 January 2024 638 6 View

How to use this type of data for scRNA-seq analysis?

Lately I'm working on the scRNA-seq analysis. It took some time for me to find a proper dataset on GEO, whose Accession ID is GSE157783. I expected to get three files of each sample from the...

29 November 2023 7,985 2 View

Is there thesis topic for MSc in Data Science ?

I am a master student. last year I started my course and now time has come to choose topic for my master thesis. I am interested in the field of natural language processing to write my...

29 September 2023 4,255 0 View

EMSA：Will the protein block the Fluorescence signal of the probe?

I'm running EMSA and add the FAM Fluorescent Groups to 5' ends of my probe. However, the cis-element of the protein in the probe is close to 5' ends(only 7bp).Will the protein block the...

17 September 2023 9,688 2 View

How to deposit negatively charged gold nanochains onto silica?

I have gold nanoparticles linked closely together by divalent ions such as Zn2+ or Cd2+ to form long gold nanochains. I was using APTES to form positively charged amine groups on the silica then...

01 September 2023 6,906 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View

What is the best way to drop a plasmid?

Please address the best way to drop a plasmid. Background: I have a "bait" plasmid resistant to kanamycin and a "prey" plasmid resistant to carbenicillin. After many rounds of streaking on...

25 July 2024 2,532 3 View

How do regenerate Amberlite XAD 4 resin for further cycles of use for removing 0.5% Sarkosyl from recombinant protein solutions?

I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...

25 July 2024 5,001 2 View

How do nutrient transporters and aquaporins interact under abiotic stress in plants?

"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...

23 July 2024 9,109 0 View

Consequences of excess bases in the MCS after digestion?

Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...

17 July 2024 2,787 4 View

My rosetta strain has chloramp resistance and i am transforming my plasmid with amp resistance but not getting colonies on chloramp/amp LB plates?

I have done the same procedure with BL21 and pLysE but i got colonies but multiple times on rosetta i did but not getting. can anyone explain what would have gone wrong?

12 July 2024 9,904 1 View

Santosh H. S. Kumar

Are you looking for protein modification or just looking for the protein expression in the western blot???

for expression studies if it is ECL u r doing u can get fairly good amount of protein in detectable concentration.

if it is for looking protein modification, it depends on the Ab u r using,

Jay Patankar

Hi Santosh, Im looking forward for western blotting but would eventually would also like to fractionate the homogenates. thanks

Aaron M Herndon

I want to know, too. I am harvesting feline islets and I am able to get roughly 600ng of RNA from 50 islets. I have the protein but have not quantified it yet. Still sitting in the -80 :)