How much H2SO4 is used for hydolysis of exopolysaccharide to characterize monosaccharide composition?

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Karen A. Darbinyan

Hello, to be sure that acid enough you can take 1g of exopolysaccharide add 10ml 0.5M Sulfuric acid. Aso you can take attention on hydolysis solution pH after addition of acid which should be not above than 2.0 for better yield.

Good luck.

Paria Rahnama

Karen A. Darbinyan thanks for your help