Has anyone tried using napthoresorcinol for the estimation of polysaccharide capsule of serotype D39 of S. pneumoniae?

18 June 2024 0 844 Report

For the estimation of carbohydrates there are different methods. As D39 serotypes contain glucuronic acid as one of the monosaccharide sugars, napthoresorcinol can be used to quantify it. if anyone has done it before please let me know.

More Shabnam Basheer's questions See All

What test is proper for detecting the relation between two nominal variables?

We have two nominal variables: diet and location. We want to know whether the diet of the species differs in various locations. I know it is possible to do it by chi-square, but I also have seen...

24 October 2020 7,162 14 View

What software do you suggest for optical simulation?

I need to simulate faraday effect

10 October 2020 2,649 3 View

Does a linear polarization of light can be decomposed into the superposition of two equal-amplitude circularly polarized components ?

what are the mathematical formulation?

27 September 2020 9,838 7 View

Q1:find to Differential Equations with the same method?

find to Differential Equations with the same method

21 September 2020 7,261 3 View

How to do confirmatory factor analysis for a retrospective pre-post test questionnaire?

I've done a pilot test with a retrospective pre-post test questionnaire to measure changing in attitude. Now I want to enter the data collected from questionnaire in SPSS to do a confirmatory...

21 September 2020 7,726 6 View

Isolation of Mouse Monocytes for ADCC?

Hi All, Immunology novice here, looking for some advice on mouse monocytes. We are looking to isolate mouse monocytes suitable to demonstrate In vitro ADCC. we tried out two methods: Mothod 1:...

17 September 2020 2,076 3 View

What is suitable sample size calculation method while using SEM?

My population is all people above 18 years old in the country, so I think this is an infinite population. Is it suitable to use Cochran formula for sample size calculation while I want to use SEM...

02 August 2020 1,029 6 View

What is the reasons to get very grainy microstructure of the steel matrix composite and particles not completely bound together?

I have a question regarding the microstructure of the steel matrix composite. When I prepared samples of stainless steel with % addition of nanoparticles of TiC using metal injection molding, this...

25 March 2020 2,588 5 View

Why the performance of ArcGIS occasionally changes when the output is not specified to be created in the default address?

Hi everybody I have noticed that sometimes while working with some commands in GIS I don't get the results I expect. Then I do the same process exactly in the same manner except for the output...

17 February 2020 5,601 9 View

LTE eNB code running on OpenWRT?

Is there any code for eNB (for LTE) that could be run on an OpenWRT Linux to emulate eNB in a test-bed environment? Please help.

18 June 2019 7,286 6 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

How to increase solubility of Poly acrylic acid in Dioxane solvent?

I am working with Polyacrylic acid (100.000Da) and instead of water, I need to use another solvent (dioxane) to dissolve PAA. However, because of the high molecular weight, it's quite hard to...

25 February 2021 2,225 5 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

Is it efficient to do liquid biopsy to investigate the gene expressions of the tumor?

To do liquid biopsy and look for specific gene expressions there are so many published papers. Thera are different protocols and procedures used to isolate circulating cell free RNA or mRNA from...

23 February 2021 7,326 5 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View