After trypsinization I have to store cells in liquid nitrogen, for that I am going to use DMSO as cryoprotectant, so 10% (v/v) or 20% (v/v) DMSO should I use, because my friend says high concentrations of DMSO is toxic to cell
you can use upto 5-10% DMSO used as a cryoprotectant in freezing medium. dont go beyond 10% it will cause toxic to the cells.
I 10% DMSO by volume as well when freezing cells.
thank you for your value points
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