Chandrasekaram Kans 430-2100

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Questions related from Chandrasekaram Kans 430-2100

How much DMSO we can add for cryostorage of mesenchymal cells?

After trypsinization I have to store cells in liquid nitrogen, for that I am going to use DMSO as cryoprotectant, so 10% (v/v) or 20% (v/v) DMSO should I use, because my friend says high...

11 November 2014 7,711 3 View

Can I use HEPES as additional pH buffer in MEM (lonza) for cultivating mesenchymal stem cells?

I am working with mesenchymal stem cells in MEM medium, can i use HEPES as additional pH buffer? because when i take it out from co2 incubator, observing colour changes. how to relove this problem

11 November 2014 1,692 10 View