A sub-question is, is it better to prepare percoll gradient with Hank's Balanced Salt Solution or with KCl? The purpose is to isolate macrophages of a fish head kidney.
You use gradient solution 1 ml each, then 6-8 ml cell suspension on the top of gradients in 15 ml falcon tube.Or you can use cell filter (stainless steel).
Thank you very much for your help Ms. Irez. Would it be correct if I put 7ml of each gradient concentration and then I load 25ml on it in a 50 ml Falcon?