Olivier Coste

2 Questions 4 Answers 0 Followers

Questions related from Olivier Coste

Any method suggestion to covalently graft an oligosaccharide on a protein?

The oligosaccharides is a sulfated rhamnoglucuronan and the protein would probably be BSA.

13 March 2014 5,802 2 View

How much cell suspension (in L15) volume may I load on a 30 ml percoll discontinuous gradient (15ml 34% and 15 ml of 51% in a 50 ml falcon tube)?

A sub-question is, is it better to prepare percoll gradient with Hank's Balanced Salt Solution or with KCl? The purpose is to isolate macrophages of a fish head kidney.

19 December 2013 2,659 3 View