I have to do oral glucose tolerance test OGTT and measure both glucose and insulin level during timepoints (0,15,30,60,120 min). I am worried regarding volume of blood collection, I assume collecting 0.1 ml at each point to get enough serum for ELISA test,( in total 0.5 ml in the same day ) , is too much for mouse and that may lead to kill mouse during the experiment!
what's the suitable amount to keep mouse alive?
In the UK, the Home Office guidelines say that the maximum volume that can be taken from a mouse is
Hi, in France we were cautious about the blood sampling during OGTT and ended up collecting blood only at t0, t30, t60, and t120 (with glycemia taken at 0,15,30,45,60,90,120). We used to take half a capillary per timepoint (1 full capillary for t0), 1 capillary represents 75 ul, and used ELISA insulin kits that require 5 ul only so we had more than enough. Even if done in duplicate, half a capillary (approx 35-45 uL) should be more than enough for you to do your analysis, 100 uL per timepoint is way too high for a mouse!
