What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

22 February 2021 6,569 3 View

What are some underexplored theories and concepts in Activated Carbon as A Water Filter?

Hi, me and my team mates are currently planning to pursue the topic of Activated Carbon from Coconut as a Water Filter. And we would like to ask for your suggestions and knowledge on what specific...

21 February 2021 7,561 4 View

What properties of water shall be analyzed and evaluated to test the effectiveness of activated carbon?

I would like to ask what other properties of water (pH and such) shall be analyzed and evaluated to test the effectiveness of activated carbon from filtering the contaminants present in the water?

20 February 2021 9,422 8 View

Who are the possible experts I can reach out to regarding the reseach topic: Activated Carbon as Water Filter?

Hi! Me and my team mates are currently new in doing a research study. We do not have any previous experiences in research. And we just wanted to know what type of experts (the field they in) can...

19 February 2021 9,596 4 View

Best loss function for special classification problem in neural networks ?

Hello, I've build a Conv1D model to classify items into 6 categories (from 0 to 5). (simplified example) I use therefore a CrossEntropy loss function that penalizes the errors with high...

19 January 2021 3,199 1 View

Anybody to help me with Code source for DOUBLE CONCAVE FRICTION PENDULUM BEARINGS Please?

Hello, would you please share the codes for Base Isolation of structures using Double Concave Friction Pendulum Bearings? I am doing a related research but as I am just a beginner in MATLAB, I...

18 January 2021 6,683 1 View

Are normality checks required for large sample sizes?

07 January 2021 6,360 2 View

Who created the NAMI Self-Care Inventory (SCI)?

The SCI questionnaire for measuring domains of self-care appears in several places on the internet and scantly in the literature. There are no published validation studies or...

02 December 2020 6,848 1 View

Has anyone used Colwell's EstimateS software to measure beta diversity of marine fishes?

I recently just found out about this statistical software and am interested to learn more about how this can be applied in marine fish ecology. Any leads on relevant literature would be most helpful.

15 November 2020 6,960 2 View

Any molecular beacon producing company to recommend ?

Hello, For one of my projects I would like to test a Live RNA imaging using molecular beacons such as in this paper : Molecular Beacon-based Detection and Isolation of Working-ty... As we...

28 October 2020 9,457 2 View

Does anyone have problems performing CRISPR KO in human primary T cells?

For the past few months, I have been troubleshooting a CRISPR KO of human CD3e in human primary T cells. I got the KO to work one time using 1250 ng TrueCut Cas9 (Thermo) + 250 ng (or 7.5 pmol)...

11 January 2021 6,016 3 View

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I am working with new fish cell lines especially from cold-water fishes, so how can we choose the best program and process to get cells with higher viability and transfection efficiency?...

16 September 2020 9,574 2 View

Does anyone have experience with transfection of Cas9 into the TMD8 cell line?

I want to transfect D10Cas9 with 2 sgRNAs to make a nickase, so I need a double+ transfection. When I transfect TMD8 with the Neon electroporation system viability drops to

31 December 2018 3,000 3 View

Does anyone have experience with transfecting Cas9 protien RNP complex to Jurkat cells?

Hi, I saw some publications that recommended Neon transfection system, but I don't have it. I do have BTX and AMAXA transfection systems.

09 October 2018 7,055 3 View

Any suggestion on choosing the nucleofector device for Jurkat transfection?

Hi all, we're currently working on T cells and considering getting a nucleofector device for highly efficient transfection yield of Jurkat T cells. Does anyone have experience about this device?...

07 August 2018 9,698 3 View

How to calculate siRNA concentration for Neon transfection ?

Hello, I am using Neon transfection system to transfect siRNA into suspension cell line. When calculating siRNA conc., does it have to based on tip volume or final volume. Although, i have an...

18 July 2018 5,766 2 View

THP-1 transfection (NEON electroporation system) condition with subsequent recovery and PMA induction ?

Dear all, I have been performing transfection of GFP-tagged plasmids into THP-1 cells using NEON electroporation system. However, the relatively low transfection efficiency suggests that I might...

06 July 2018 1,019 2 View

How to calculate siRNA concentration for Neon transfection ?

Hello, I am using Neon transfection system to transfect siRNA into suspension cell line. When calculating siRNA conc., does it have to based on tip volume or final volume. Although, i have an...

06 July 2018 8,878 3 View

Has anyone tried to transfect sup-b15 cell line using recombinant cas9?

Hello everyone, I am starting a project in which I'm planning to create a cell line with a specific deletion. I am going to use SUP-B15 and I need to know if anyone has ever transfected this cell...

06 July 2018 4,159 1 View

Optimal electroporation conditions for MDAM 468 cells using the Neon device?

I am trying to transfect MDAM-468 breast cancer cells with GFP through electroporation. I tried electroporating 50,000 cells in a 24-well plate with 2ug GFP and 1400 Volts, 10ms, 4 pulses (as...

06 July 2018 6,482 1 View