I was wondering how this system compares to the BioRad electric pulse system for delivery of plasmid components? what is the optimized buffer or what is your advice to imorove KD efficiency?
I have a question about zinc chloride salt powder. When I put zinc chloride powder in an oven at a temperature of 50 degrees to dry it (because it may absorb moisture), after about an hour, the...
26 November 2020 3,751 3 View
I want to know what kind of microscope should I use to check the stability of dual emulsions (W/O/W)? upright or invert? Fluorescence or polarized or ordinary light? What field of vision should I...
12 November 2020 6,044 3 View
Hi everybody. I want to simulate a super molecule in a solvent like heptane in material studio. Solvent molecules are about 700 and are around the super molecule. How can I simulate these...
07 September 2018 7,643 4 View
when we prepare a hydroalcoholic extractof a plant, after evaporation in vacuum condition, we do not have a completely dried extract. What do you do for completely drying these extracts?
28 January 2018 8,328 2 View
I appreciate any information regarding your experience with dilute suspension (
14 January 2014 7,192 7 View
We want to protect the S of methimazole, does anyone have any suggestion?
22 November 2013 4,267 7 View
What is your idea about "negative intercept" in calibration curve in HPLC? Can you recommend a good book on this subject?
08 March 2013 485 4 View
Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...
03 March 2021 1,499 2 View
Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...
03 March 2021 6,299 3 View
Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...
01 March 2021 210 1 View
Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...
01 March 2021 2,215 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...
01 March 2021 9,310 4 View
I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...
01 March 2021 9,015 2 View
I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...
01 March 2021 6,142 3 View
I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...
28 February 2021 5,440 3 View
I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...
28 February 2021 4,949 3 View