I was wondering how this system compares to the BioRad electric pulse system for delivery of plasmid components? what is the optimized buffer or what is your advice to imorove KD efficiency?
Dear Friends I hope you are well. "I'm interested in the concept of a 'Knowledge Graph of the Cold Chain.' If you have any files or information on this topic, could you please share it with me?...
05 March 2024 2,305 2 View
Can passive movement of a joint, for example plantar/dorsiflexion of ankle joint, elicit EMG activity in tibialis anterior and gastrocnemius muscles?
09 June 2023 8,007 4 View
Hi How to measure the concentration of free calcium in the cytoplasm of rat beta cells? Thanks
15 April 2023 3,122 7 View
Why chitosan glutaraldehyde hydrogel does not swell and absorb water after being placed in the refrigerator?
11 March 2023 1,607 3 View
Does anyone have experience with using Amaxa nuclefactor or Neon for electroporating CRISPR plasmids or any other vectors in primery cells?
28 February 2023 6,834 2 View
Hello. Good Night Recently in class, students were asked a question that is important to me, and I heard some interesting answers. My question is, how can a woman perform many duties and...
05 December 2022 6,891 8 View
I have two small characteristic peaks in the NMR spectrum showing negative integration values. I would appreciate it if you could share your opinion.
28 November 2022 9,933 2 View
Sohrab Sepehri one of Persian poets says at the end of one of his tender and mystical poems called "water`s footsteps" : It is not our business to fathom the mystery of rose🌹 Perhaps our...
26 November 2022 6,979 90 View
I want to have an in vivo experiment in one lab and cryosectioning and SA-b galactosidase staining in another lab, for long-distance transportation of mice kidney samples, Does anybody have...
19 August 2021 3,603 0 View
I have a question about zinc chloride salt powder. When I put zinc chloride powder in an oven at a temperature of 50 degrees to dry it (because it may absorb moisture), after about an hour, the...
26 November 2020 3,919 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?
07 August 2024 4,616 0 View
Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...
06 August 2024 5,373 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View