Stephan Spangenberg

6 Questions 20 Answers 0 Followers

Questions related from Stephan Spangenberg

Can qPCR be successfully performed with low starting quantities of RNA (less than 100 ng)?

I have total RNA extracted from samples which are rather precious. I am trying to perform cDNA synthesis then qPCR. The RNA appears to be intact and reasonably pure, but I only have between 20 and...

25 March 2019 7,525 4 View

Are antifungals safe to use with iPSCs or ESCs?

I use antimycotics such as Amphotericin to protect some of my cell cultures. I would like to use them with iPSCs, but I understand many cells are negatively affected by antimycotics. Has anyone...

07 May 2016 7,994 5 View

Has anyone been able to use a Nikon DS-Fi1 U2 microscope camera with non-Nikon software?

My lab has a Nikon DS-Fi1 camera with a U2 controller. We have the free NIS-Elements F software but we really need some of the capabilities of NIS Elements BR, such as movie making, but the $3000...

15 September 2015 3,386 3 View

Can CRISPR/Cas9 Knockout be estimated by Western Blot before clonal isolation?

I have a CRISPR/Cas9 Wild Type (pSpCas9(BB)-2A-GFP (PX458)) plasmid that I have prepared, targeted for a nuclear protein's gene. I have already confirmed its sequence. I would like to test if it...

02 October 2014 2,182 4 View

What is the optimal amount of Lipofectamine 3000 to use with 293T cells?

I would like to transfect 293T cells with Lipofectamine 3000. This is the first time I will have used Lipofectamine 3000, previously I only used Lipofectamine 2000, and I am wondering if anyone...

28 July 2014 4,474 6 View

Why is my restriction digest producing overly large transformants?

I cut a MSCV vector with NotI and SalI. The vector is 6kb long and the restriction sites are about 100 base pairs apart. Upon performing gel electrophoresis, the cut vector produced a clear,...

01 June 2014 7,543 5 View