Dear Mohammed: Your question is too simple. It depends on the testing conditions. Acute exposure? (minutes) long term? hours, days? What is the objective of your study? Compare a substance with a standard? Compare different samples. Just test one compound? The point is not at what concentration the compound is toxic but at what concentration the compound produces cell damage that can be distinguished from the normal cell damage or dying in culture. What is the control cell death +- standard deviation or standard error. From those values you can define analytically the concentrations that are significantly different from the control. I think you can say that those cocnentrations have some "toxicity"
Thanks dr Reyes.....I heard that the dose that reduces cell viability to 85% is not toxic..Some scientists said dose of compound reduce cell visibility to 80 % is nun-toxic even the difference is statistically is significant and there is a significant apoptosis and DNA damage. No body of them provided a references
Mohammed: Define your objectives and forget opinionologists. Toxicity is a too wide term to dwell into it. Do you want to lyse 100% of your cells? Is 80% or less Ok and stop growth (that would be a nice cancer arrest compound?)? No rules. Just criteria of waht you are looking for in your compound.