I have been reading that people are storing cells at -80C, but I have -20C refrigerator. Is it possible to store bacterial culture at -20C?
Hi Suparna,
I stored bacterial culture at -20C. of course the longer stored -80C but also -20C to store bacterial culture not a problem.
Good luck..
I agree with Azer. there is no problem with storing Bacterial culture in -20. If I were you I will try to renew the cultures from time to time.
Good luck
You can store your bacterial sample at -20 C with Glycerol for a longer time (months). But same at -80C for years.
Thanks to all of for your response.
I will try with glycerol definitely.If its months, ...not a problem.better to renew it at intervals.
Good Luck ....
Thanks Alicia,...your info. is interesting. May be I can try that with Pseudomonas also..
Good luck
It depends of course on the bacterial species. But storing the tubes for a few years at minus 20 degrees is not normally a problem. I recommend strongly. however, to prepare several parallel tubes in the beginning and not to re-freeze those that you have used already. - Good luck!
Thank you Sir for the info you provided.
Myself from Physics and now delving into microbiology, for my biomedical project from the purview of Physics, is having a many info from the internet, apart from standard books.Most of the time they are very helpful.
So thank you..
and additionally glycerol is a cryoprotectant. At sub zero temperatures, it will ensure that ur microbes wont undergo disruption due to formation of ice crystals. They'll stay healthy. Have a great day.
For long-term storage of bacterial isolates, please check out:
http://aem.asm.org/content/52/2/388.full.pdf
https://www.google.ps/search?q=methods+of+storage+of+bacterial+isolates&oq=methods+of+storage+of+bacterial+isolates&aqs=chrome..69i57.28344j0j8&sourceid=chrome&espv=210&es_sm=93&ie=UTF-8#q=methods+of+long-term+storage+of+bacterial+isolates
Dear Suparna dutta sinha,
You should keep bacteria in broth medium with glycerol and keep in -80 C that it 's OK more than -20 C because the ice crystral in -80 C is a smaller than ice crystral in -20 C.
Based on my experiences at Microbial Collection of Pasteur Institute of Iran, I prepared BHI broth or TSB for fastidious bacteria and LB or Nutrient broth for others. Media prepared by 20% glycerol. By this method we stored our bacteria for 1-2 years. But for long time storage bacteria must be lyophilized and store at -80.
Grow your pure culture on an appropriate solid medium, scrape it off with a loop and
suspend small clumps of the culture in sterile glycerol,distribute in quantities of 1–2 ml in screw-capped tubes or vials, seal the top it with a paraffin tape,
Store at -20 °C.
Avoid repeated freezing and thawing. Transfer after 12–18 months.
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