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Questions related from Reza Azizian
I isolated phage against Pseudomanas aeruginosa PAO from sewage. There is a need to have sufficient pellet for SDS-PAGE. I recruited PEG 8000 (40% PEG+ 0.5M NaCl ) in equal volume for...
05 May 2014 4,196 3 View
I isolated phage against MRSA, and took a micrograph by TEM. My phage is a member of Siphoviridae. I tried centrifuging PEG 6000 (8%) at 10,000rpm for 15min but there wasn't an appropriate pellet...
03 March 2014 2,311 2 View
I have done PCR at different annealing temperatures and used Norgene Kit to extract DNA. There is a just a band upper ladder in all of gels. I checked my whole DNA (before PCR) by...
10 October 2013 8,336 8 View
I have done SDS-PAGE for P.aeruginosa and its phage. I prepared 12% Gel and used 11-258 KD marker (Genedirex). There is a sharp band for marker but there aren't any bands for phage or bacteria. I...
06 June 2013 4,743 2 View
I stored them by glycerol 40% which equally added to LB broth. They had function after 2 months but now after 4 months they don't have ability to produce plaque on plate.
02 February 2013 4,701 5 View
