Could someone please tell me how to start modelling proteins?

More Suparna Dutta Sinha's questions See All

Anyone working on genetic expressions of Pseudomonas aerignosa, subjected to stress?

genetic expressions are vital tools for understanding the behavior of microbes, and they get altered when the bacteria are subjected to different stress. Pls suggest how to proceed.I would be...

08 September 2014 7,223 2 View

Anybody working on genetic expression of Pseudomonas aeruginosa in biofilms?

The genetic expression is an important factor of bacteria in biofilms.

08 September 2014 7,870 1 View

How long is it possible to store bacterial culture at -20 C?

I have been reading that people are storing cells at -80C, but I have -20C refrigerator. Is it possible to store bacterial culture at -20C?

02 March 2014 5,443 17 View

Can anybody explain the difference in EPS of Pseudomonas aeruginosa and Staphylococcus aureus?

The EPS of Pseudomonas and Staphylococcus seems to be different in appearance.

02 March 2014 6,120 3 View

Can anybody tell me how to scale poly-methyl methacrylate, high density polyethylene, and polytetrafluoroethylene according to their hydrophobicities?

All these materials are in general known as hydrophobic materials. We have a scale to measure the hydrophobicities of amino acids. Does such a scale exist for these polymers?

02 March 2014 6,698 2 View

Can anybody refer a group working on bacterial adhesins?

I am interested in bacterial adhesins and want to know about a group working on it.

02 March 2014 7,532 3 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Is it necessary to covary exogenous constructs in a structural model?

I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...

03 August 2024 6,028 4 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View