if synthesized oligos are transfected into cells (in vivo), how long will they be stable? Are they going to be degraded within hours, days or weeks? Any information/references will be highly appreciated.
We obtained pLKO5-EFS-GFP from addgene (Ben Ebert Lab) which contains U6-gRNA-EF1s-GFP. Using transient transfection, we got very good transfection. With virus, the GFP expression is extremely...
06 July 2016 9,370 4 View
I did not find any resource.
05 June 2016 7,692 3 View
How is the performance? As compared to virus? My plan is to deliver oligos in cortex
05 June 2016 6,886 2 View
I am trying to subcloning the short EF1a promoter to drive Cas9 in neurons but not sure if it works as well as CAG promoter. Anybody has experience with EF1a in neurons?
02 March 2016 4,545 5 View
I am looking for a good and simple protocol to stain growth cone in cultured neurons but are confused by many different fixative and markers. Any protocol or article?
01 February 2016 8,199 6 View
I am planning to express a gene(dCAS9) in neurons with CAG promoter based vector but only have a vector with CMV promoter with CAG enhancer. Are they the same regarding the expression efficiency...
31 December 2015 1,246 4 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
to identify themes in question with APA style references
07 August 2024 2,239 5 View
During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...
06 August 2024 726 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...
30 July 2024 6,648 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?
29 July 2024 3,229 1 View