Anybody used jetpei in vivo transfection in brain?

More Yu Wang's questions See All

What is wrong with the expression from pLKO lentivirus?

We obtained pLKO5-EFS-GFP from addgene (Ben Ebert Lab) which contains U6-gRNA-EF1s-GFP. Using transient transfection, we got very good transfection. With virus, the GFP expression is extremely...

06 July 2016 9,370 4 View

Is there any transgenic inducible CAS9 rat available?

I did not find any resource.

05 June 2016 7,692 3 View

How long does synthesized oligos last in cells?

if synthesized oligos are transfected into cells (in vivo), how long will they be stable? Are they going to be degraded within hours, days or weeks? Any information/references will be highly...

05 June 2016 6,811 0 View

Is EF promoter working well in neurons?

I am trying to subcloning the short EF1a promoter to drive Cas9 in neurons but not sure if it works as well as CAG promoter. Anybody has experience with EF1a in neurons?

02 March 2016 4,545 5 View

Any good protocol for filopodia/growth cone staining?

I am looking for a good and simple protocol to stain growth cone in cultured neurons but are confused by many different fixative and markers. Any protocol or article?

01 February 2016 8,199 6 View

CAG promoter and CMV promoter with CAG enhancer are the same?

I am planning to express a gene(dCAS9) in neurons with CAG promoter based vector but only have a vector with CMV promoter with CAG enhancer. Are they the same regarding the expression efficiency...

31 December 2015 1,246 4 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

How to start a Molecular Dynamics Simulation?

Is it possible to conduct a molecular dynamics simulation to see the effects of a specific carbohydrate on the structure of lipids (e.g., micelle structure)? I am a beginner in this field and plan...

03 August 2024 3,371 3 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View

Which will be the best software for the Hydration shell analysis with molecular dynamics?

I am using a windows system, what software I should use for hydration shell analysis with molecular dynamics?

02 August 2024 3,143 4 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Issues with Protein Expression in Transfected cells?

Hello I am trying to create a stable cell line in HEK293 via Lipofectamine 3000 transfection. My plasmid is a CD63-IL10-GFP construct with Puromycin resistance. I am successful with the...

30 July 2024 6,648 1 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

Does anyone have experience electroporating SKOV3 cells with a large plasmid using Neon transfection?

I have been trying to electroporate SKOV3 cells with a large plasmid (11kb) without much success. Any tips?

29 July 2024 3,229 1 View

Cédric Sapet

Dear Yu Wang,

I would like to offer you the possibility to try our reagents as an alternative for your application. Two strategies may be used:

either Magnetofection: our in vivo line of magnetic reagents are designed for in vivo targeted transfection and transduction. This combines magnetic nanoparticles and nucleic acid vectors that are retained after injection at the magnetically targeted site. In this way, systemic distribution is minimized and toxicity is reduced.

or "classic" transfection reagents that are really efficient in vivo.

Results have been obtained in Rat brain for DNA transfection using the methods presenting above.

Would you like to try them, please do not hesitate to contact me via ResearchGate or directly at [email protected];.

best regards,

Cedric

Dear Yu,

Do you have some achievement to share regarding in vivo transfection in brain ? I would be very glad to offer you the possibility to try one of our latest reagents.

please do not hesitate to contact me,