Yu Wang

7 Questions 4 Answers 0 Followers

Questions related from Yu Wang

What is wrong with the expression from pLKO lentivirus?

We obtained pLKO5-EFS-GFP from addgene (Ben Ebert Lab) which contains U6-gRNA-EF1s-GFP. Using transient transfection, we got very good transfection. With virus, the GFP expression is extremely...

07 July 2016 9,331 4 View

Anybody used jetpei in vivo transfection in brain?

How is the performance? As compared to virus? My plan is to deliver oligos in cortex

06 June 2016 6,851 2 View

How long does synthesized oligos last in cells?

if synthesized oligos are transfected into cells (in vivo), how long will they be stable? Are they going to be degraded within hours, days or weeks? Any information/references will be highly...

06 June 2016 6,763 0 View

Is there any transgenic inducible CAS9 rat available?

I did not find any resource.

06 June 2016 7,638 3 View

Is EF promoter working well in neurons?

I am trying to subcloning the short EF1a promoter to drive Cas9 in neurons but not sure if it works as well as CAG promoter. Anybody has experience with EF1a in neurons?

03 March 2016 4,494 5 View

Any good protocol for filopodia/growth cone staining?

I am looking for a good and simple protocol to stain growth cone in cultured neurons but are confused by many different fixative and markers. Any protocol or article?

02 February 2016 8,149 6 View

CAG promoter and CMV promoter with CAG enhancer are the same?

I am planning to express a gene(dCAS9) in neurons with CAG promoter based vector but only have a vector with CMV promoter with CAG enhancer. Are they the same regarding the expression efficiency...

01 January 2016 1,194 4 View