How long does it take to polarize macrophages into M1/M2?

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Mothana Ali Khalil

nice question I Follow Answer

Anna C Belkina

it It really depends on your system. What's your starting material (cell origin, species)?

Megan Marlow

Rat bone marrow

If your conditions are polarizing to begin with (i.e. you differentiate and polarize at the same time) then 6-7 days are plenty. If you first differentiate then polarize then it's 2-3 days (usually).