How long can poured MRS plates sit at room temp before placing in refrigerator?

More Madison paige Wagoner's questions See All

How long does a Poly-O/ Laminin Coating last on a FlexCell Collagen plate?

My lab routinely coats the FlexCell Collagen plates with Poly-O followed by laminin for neural stem cell plating. Does anyone know how long these coated plates can be stores before use?

07 March 2024 6,973 0 View

Is there a measurement scale for assessing one's self-perception of their influence on others?

I see many scales for assessing one's susceptibility to social influence but am having trouble finding one that assesses how influential on others one believes themself to be.

20 February 2024 9,616 3 View

Decreased LC3A/B output in Westerns with >MOI2 HSV-1?

Has anyone else run Westerns for LC3A/B with HSV-1 infections in human and mouse glial cells and seen little to no LC3A/B expression with viral MOI above 1? I used MOI 1, 2, and 5 a few times and...

24 January 2024 8,310 0 View

Has anyone ever frozen their standard gradient and reused it for subsequent ELISAs and compared results to test if reusing frozen standards works?

I had extra solution from my standard gradient vials after plating my standard gradient in my ELISA wells and was wondering if it was reusable for another hAST IL-8 ELISA? Has anyone seen similar...

18 October 2023 4,962 1 View

Has anyone else seen plastic degradation in the cap of their 1xPBS/0.05% PBS tween 20 storage container?

I was running an ELISA today and noticed there were chunks of plastic from the plastic cap of my 1xPBS/0.05% PBST bottle in the solution. Has anyone else seen this and did it affect your plate washes?

18 October 2023 349 1 View

Technologies Being Enabled For SLM/SLS Manufacturing In-Space?

I am currently working on a research project for developing a novel additive manufacturing system that uses selective laser melting (SLM) coupled with Fourier Transform Infrared (FTIR)...

19 September 2023 1,323 2 View

Why is Kraken2 host removal with a custom database generating .fastq files with spurious "x"s?

I'm using Kraken2 to generate a custom database to run a shotgun metagenomics (microbiome) dataset against for associated host removal (the custom database is the host). The database builds fine,...

24 October 2022 6,953 7 View

Has anyone had issues with pentylenetetrazol (PTZ) kindling in male mice?

I am currently working with 8 week old male C57BL/6 mice from Charles River. I am administering 35mg/kg PTZ every other day for the last 12 days (6 PTZ administration days) and have observed...

13 June 2022 9,211 0 View

What do you need to do to have a marantz model PMD661 pick up on bat echolocations?

I am trying to use the Marantz model PMD661 to measure bat activity, but have been unsuccessful at attaining any data using the model, despite being able to see the bats present. I was hoping...

26 May 2022 907 1 View

How to prepare PVA crosslinked with glutaraldehyde to use for spin coating films?

Hello, I really have no experience with how to prepare PVA/GA films. These films will need to be spincoated to be ~200-500nm thick. I have PVA (avg weight 85,000-124000, 87-89% hydrolyzed) and...

08 June 2021 1,594 2 View

Feedback defines the constitution of an organism?

“Here is a thought experiment. Let's place Rodolpho Llinas's jarred-brain on top of a body (Fig. 1). I bet Llinas would argue that his jarred-brain retains its own consciousness, and the android...

11 August 2024 2,483 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Can you recommend a 96-well plate for minimal cell adherence to the wells during phagocytosis experiments?

I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...

04 August 2024 5,228 4 View

Swimming/space travel depends on the proprioceptive muscle spindles?

When the entire neocortex is ablated in rodents, although they are still able to swim, all the limbs move continuously and asynchronously (Vanderwolf 2006; Vanderwolf et al. 1978). Normal animals...

03 August 2024 835 3 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

Found this object in the cell culture - do you have any suggestions what it might be?

Dear researchers, Today, we found this object in one of the wells of a 96wp culture plate. The plate contained MEC-1 cells and GelTrex. I am quite curious what this is (because it's not cells). Do...

01 August 2024 2,250 2 View

Publishing a checklist?

Does anybody know of entomological Journals without page charges that would publish an extensive checklist (Hong Kong Aculeates wasps) covering 350 species (of which more than half are new to Hong...

01 August 2024 6,134 2 View

How to make overlapping p-h diagram in EES?

I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...

30 July 2024 647 7 View

Do some Staphylococcus aureus strains have in vitro antimycobacterial activity ?

Some Staphylococcus aureus strains Inhibit the growth of Mycobacteria in Mueller Hinton Agar medium containing 10% OADC. Do some Staphylococcus aureus strains have in vitro antimycobacterial activity?

29 July 2024 10,023 2 View

Stoica Costin

Let them stay overnight in the place where you poured e.g. laminar flow cabinet (turned off)

Fatemeh Bagheri

The culture medium becomes solid, increasing with time depending on the thickness of the medium

It is best to refrigerate immediately after solidification

Siyu Chen

Can store for a long time, at least 2 weeks at room temperature. But a long time outside the fridge results in super dry agar.