Has anyone ever frozen their standard gradient and reused it for subsequent ELISAs and compared results to test if reusing frozen standards works?

19 October 2023 1 5K Report

I had extra solution from my standard gradient vials after plating my standard gradient in my ELISA wells and was wondering if it was reusable for another hAST IL-8 ELISA? Has anyone seen similar or weaker results?

Erin Leigh Mills

Hi Madison! I have saved my ELISA standard for mouse IL-6 in the -80 several times without seeing any weakening in the signal. The longest I have saved a standard, however, was only for a couple of weeks (and only thawing it 2 times total). I'm sure storage temperature and freeze-thaw cycles play a role. Hope this helps!

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