Decreased LC3A/B output in Westerns with >MOI2 HSV-1?

25 January 2024 0 8K Report

Has anyone else run Westerns for LC3A/B with HSV-1 infections in human and mouse glial cells and seen little to no LC3A/B expression with viral MOI above 1? I used MOI 1, 2, and 5 a few times and noticed a large decrease with higher concentrations of the virus. I've been reading some papers on autophagy and it looks like there are some mixed results.

Badges
Science topic

More Madison Belk's questions See All

How long does a Poly-O/ Laminin Coating last on a FlexCell Collagen plate?

My lab routinely coats the FlexCell Collagen plates with Poly-O followed by laminin for neural stem cell plating. Does anyone know how long these coated plates can be stores before use?

07 March 2024 6,973 0 View

Is there a measurement scale for assessing one's self-perception of their influence on others?

I see many scales for assessing one's susceptibility to social influence but am having trouble finding one that assesses how influential on others one believes themself to be.

20 February 2024 9,616 3 View

Has anyone ever frozen their standard gradient and reused it for subsequent ELISAs and compared results to test if reusing frozen standards works?

I had extra solution from my standard gradient vials after plating my standard gradient in my ELISA wells and was wondering if it was reusable for another hAST IL-8 ELISA? Has anyone seen similar...

18 October 2023 4,962 1 View

Has anyone else seen plastic degradation in the cap of their 1xPBS/0.05% PBS tween 20 storage container?

I was running an ELISA today and noticed there were chunks of plastic from the plastic cap of my 1xPBS/0.05% PBST bottle in the solution. Has anyone else seen this and did it affect your plate washes?

18 October 2023 349 1 View

Technologies Being Enabled For SLM/SLS Manufacturing In-Space?

I am currently working on a research project for developing a novel additive manufacturing system that uses selective laser melting (SLM) coupled with Fourier Transform Infrared (FTIR)...

19 September 2023 1,323 2 View

Why is Kraken2 host removal with a custom database generating .fastq files with spurious "x"s?

I'm using Kraken2 to generate a custom database to run a shotgun metagenomics (microbiome) dataset against for associated host removal (the custom database is the host). The database builds fine,...

24 October 2022 6,953 7 View

Has anyone had issues with pentylenetetrazol (PTZ) kindling in male mice?

I am currently working with 8 week old male C57BL/6 mice from Charles River. I am administering 35mg/kg PTZ every other day for the last 12 days (6 PTZ administration days) and have observed...

13 June 2022 9,211 0 View

What do you need to do to have a marantz model PMD661 pick up on bat echolocations?

I am trying to use the Marantz model PMD661 to measure bat activity, but have been unsuccessful at attaining any data using the model, despite being able to see the bats present. I was hoping...

26 May 2022 907 1 View

How long can poured MRS plates sit at room temp before placing in refrigerator?

Hi all, I have seen a variety of techniques and wanted to see what has worked best for anyone. I have poured MRS agar into plates, so I am curious on how long they can sit at room temperature...

13 February 2022 7,760 4 View

How to prepare PVA crosslinked with glutaraldehyde to use for spin coating films?

Hello, I really have no experience with how to prepare PVA/GA films. These films will need to be spincoated to be ~200-500nm thick. I have PVA (avg weight 85,000-124000, 87-89% hydrolyzed) and...

08 June 2021 1,594 2 View

How can I prepare virus for a TEM or SEM imaging?

I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?

11 August 2024 3,115 1 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

Which is more important: The human factor or the technological factor in combating climate change?

On a personal note, even though technology has attracted a lot of interest and funding to combat climate change, it is becoming more and more clear that taking care of the "Human Dimension" is...

02 August 2024 6,773 12 View

Tissue homogenization in mouse with FastPrep-24™ 5G - is the D matrix system useful?

I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...

31 July 2024 9,186 0 View