I was running an ELISA today and noticed there were chunks of plastic from the plastic cap of my 1xPBS/0.05% PBST bottle in the solution. Has anyone else seen this and did it affect your plate washes?
It can happen if the tube is old, and/or liquid is stored for an extended period of time - better change solutions every week/month or so, depending on usage. Likely it will not affect plate wash unless the amount of plastic is very extensive, just prepare new PBS in a new bottle.