I wanted to know if we can store frozen cells in -80 C refrigerator. These cells are stored in liquid nitrogen tank (-200 C) as of now. But as liquid nitrogen vaporizes, we need to keep refilling the tank every two months. Since, the university is going to be under lockdown due to COVID-19, we will not be allowed to enter labs or be able to order new liquid nitrogen. So will it be feasible to store these frozen cells at -80 C for the coming 3-4 months?
Look at this Article.
Article Long-term Cryopreservation of Human and other Mammalian Cell...
Have a look at this manuscript:
Long-term Cryopreservation of Human and other Mammalian Cells at −80 °C for 8 Years. by Yoshitaka Miyamoto, Masashi Ikeuchi, Hirofumi Noguchi, May 29, 2018 https://doi.org/10.1177/2155179017733148
Yes, in one lab, we were keeping frozen cells at -80 or -86 or -70C. I was able to thaw and culture without ay problem.
Look at this Article.
Article Long-term Cryopreservation of Human and other Mammalian Cell...
Hi Adity,
I think you can keep your cells in -80 for several years. However I strongly suggest that after the Covid-19 situation, cultivate your cells again and prepare your nitrogen source from fresh culture. Do not put them directly into Nitrogen Tank.At least make an experiment first. For example, put some cryovials into liquid nitrogen and after couple of days, cultivate them. If they are alive you can transfer your cells into liq. nitrogen tank too.
The first concern for me is contamination.
Second, depen on my experiences, I realized that cells dye if they stay first too long in -80 and consequenlty transfered to nitrogen. With too long, I mean more than 2 months.
Cells are normaly alive -80 for years. I agree with that. However, if you put these cells from -80 after 3 months into liquid nitrogen, unfortunately cells may dye. That occured to us several times. That's why we never keep our cells more than 3 weeks in -80 after crypreservation protocol. If we forget them in -80, we use them but never put them into nitrogen tank again.
I hope your cells will be Ok.
Good luck!
Cigdem
I doubt very much the possibility if long term preservation at -80. You have to keep your liquid nitrogen
Most labs cryo-preserve cells in liquid nitrogen whose temperature reaches -190 centigrades; for those labs not equipped with liquid nitrogen jars, cells are also preserved at -80 centigrades permenantly.
Hi Adity,
I think you can keep your cells in -80 for maximum upto 8-9 months. However after that it was able to thaw and culture cells but the viability is reduced. I suggest you that after the situation, cultivate your cells again and prepare your store for nitrogen from fresh culture.
Good luck.
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