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Questions related from Adity Pore
I fix blood cells on slides using 4% PFA for 20 mins at RT. I always keep an extra slide in storage, in case if I need to re-stain the slides. So far, I have been storing my slides at 4C in a...
12 April 2021 2,010 7 View
I am currently storing my fixed slides at 4 C. These slides tend to dry out. Are these slides still good to use? can these slides be revived by adding liquid? I have attached some images .
23 June 2020 7,204 5 View
There are several EMT markers in cancer, such as vimentin, n-cadherin, SNAIL, TWIST, ZEB etc. How do you decide which one to use?
14 June 2020 9,063 2 View
I have been using conjugated antibodies for staining cancer cells. And I try preserving the fluorescence signals using Prolong antifade with Dapi. However, after 24-48 hours, the only signal I see...
10 June 2020 4,833 1 View
For example CD44 is a stem cell marker, whereas Vimentin is an EMT marker. Mesenchymal cells are called MSCs (Mesenchymal Stem Cells), then why are there different markers for stem cells and...
02 June 2020 2,769 5 View
I wanted to know if we can store frozen cells in -80 C refrigerator. These cells are stored in liquid nitrogen tank (-200 C) as of now. But as liquid nitrogen vaporizes, we need to keep refilling...
24 March 2020 8,938 10 View
I want to make a hydrophobic microfluidic device, I plasma bonded the device on a PDMS slab. Since I used plasma treatment, I wanted to know how long will it take for PDMS to lose hydrophilicity.
06 February 2020 9,598 8 View
If I have more number of foci, does it represent nuclear damage or nuclear repair? Some cells show pan nuclear staining, does it mean the cell underwent apoptosis? I am new to this area. Any...
10 November 2019 5,436 5 View
I use 4 % PFA to fix my cells on a coverslip. How long should the cells be treated with PFA? Also, how should I maintain these fixed slides for longer periods? Are these slides stored with some...
07 October 2019 2,992 6 View
My cells are fixed on a coverslip and are stained with DAPI, Cytokeratin and CD45. What steps should I take after staining the cells?
19 September 2019 1,546 4 View
If I have cells on a glass slide and I put 4% PFA on it, are the cells going to be stuck on that particular position always? Will the cells move if I add PBS to it? What exactly does fixation do...
19 March 2019 5,806 3 View
I saw small dust like particles floating in my cell culture media (DMEM) and in the cell culture flask as well. My cells are growing fine in this media. Also, I see the same particulate matter in...
08 June 2018 3,336 7 View
Co is the initial concentration of the dye, while Cn is the concentration of the dye after dilution. Intensity of the images are found using ImageJ as well as MATLAB, there is hardly any...
20 June 2017 3,253 3 View
Hi All, I am learning how to do immunostaining. Can you please share some established protocols for immunostaining breast cancer cells like MCF-7? How do we decide which primary and secondary...
01 January 1970 5,926 3 View
