What is the reason for the lack of DNA bands in gel electrophoresis?

I am working on self-incompatibility by using PCR on Prunus, but I have not yet been able to detect the DNA bands in gel electrophoresis (figure below). I replaced the master mix and the primers...

31 December 2019 430 3 View

What is the reason for decreasing the score in Researchgate?

What is the reason for decreasing the score in RG? For example, despite the fact that I regularly add research items, my citations are raised and I ask questions or answer questions, but each...

11 December 2018 5,953 8 View

What is the cause of this apple fruit disorder?

What is the cause of this color change in apple fruit?

07 August 2018 5,617 1 View

How can scientific findings from research and student theses be commercialized?

Many M.Sc and Ph.D student theses are being conducted annually in the field of agriculture and horticulture, but nobody uses their scientific findings. How can theses be applied and commercialized?

05 June 2018 3,608 4 View

How to collaborate with researchers from other countries?

Despite the problems that exist in scientific relations between researchers in developing countries, how do you think researchers collaborate together? For example, I am very interested in...

05 June 2018 7,993 6 View

What is the cause of browning of apple fruit skin in storage?

The apple fruits bought from the market after a few days in the refrigerator changed their skin color from red to almost brown, while the fruit was healthy and no signs of a pathogen were seen,...

03 April 2018 8,425 10 View

Has anyone studied the cytogenetic of Lepidium species?

Hello Does anyone have experience on karyotype, banding or flow cytometry of Lepidium species?

01 February 2018 7,750 0 View

Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference?

Dear colleagues Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference, and how do nutrition, light and growth regulators affect it?

01 February 2018 1,616 0 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

No title

01 March 2021 3,905 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

What test should I use to compare two or three means, if data is not normally distributed but sample size is > 30 in each group?

I personally feel this as a most confusing question. As per my understanding, if sample size is > 30, and sample is skewed, we can still use paramteric approach, as it will follow Z...

01 March 2021 1,587 6 View

Is it necessary to report effect size (ES) in statistics of biomedical research given that we already report p values routinely?

Pls share your thoughts

01 March 2021 7,330 4 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why there are two peaks while using the mie plot of Silver nanoparticle?

Hi, I am trying to learn the theoretical modelling of silver nanoparticle using the Mie scattering theory. After that, I discovered a software named Mieplot developed by Philip Laven, where it...

25 February 2021 7,493 6 View

How to calculate Z (the number of molecules in a unit cell) for calculating theoretical density?

I am trying to calculate the theoretical density of my ceramics materials. I use the formula below to calculate the theoretical density: Theoretical density = (Molecular weight x No. of molecules...

24 February 2021 3,823 2 View