What is the reason for the lack of DNA bands in gel electrophoresis?

I am working on self-incompatibility by using PCR on Prunus, but I have not yet been able to detect the DNA bands in gel electrophoresis (figure below). I replaced the master mix and the primers...

31 December 2019 430 3 View

What is the reason for decreasing the score in Researchgate?

What is the reason for decreasing the score in RG? For example, despite the fact that I regularly add research items, my citations are raised and I ask questions or answer questions, but each...

11 December 2018 5,953 8 View

How can scientific findings from research and student theses be commercialized?

Many M.Sc and Ph.D student theses are being conducted annually in the field of agriculture and horticulture, but nobody uses their scientific findings. How can theses be applied and commercialized?

05 June 2018 3,608 4 View

How to collaborate with researchers from other countries?

Despite the problems that exist in scientific relations between researchers in developing countries, how do you think researchers collaborate together? For example, I am very interested in...

05 June 2018 7,993 6 View

What is the cause of browning of apple fruit skin in storage?

The apple fruits bought from the market after a few days in the refrigerator changed their skin color from red to almost brown, while the fruit was healthy and no signs of a pathogen were seen,...

03 April 2018 8,425 10 View

Has anyone studied the cytogenetic of Lepidium species?

Hello Does anyone have experience on karyotype, banding or flow cytometry of Lepidium species?

01 February 2018 7,750 0 View

Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference?

Dear colleagues Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference, and how do nutrition, light and growth regulators affect it?

01 February 2018 1,616 0 View

How large is the genome size of the Lepidium draba?

I got the the genome size of Lepidium draba using flow cytometry technique, but since I do not know about the size of its genome (1C or in meiosis), I can not determine the ploidy level. How large...

31 December 2017 4,668 2 View

How can I improve the concentration of banana fruits extracted RNA?

Currently, I'm working with banana fruits. I've tried to do RNA extractions with different methods, and I've gotten total RNA without contamination, but I couldn't get a high concentration of...

22 February 2021 6,126 2 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Does calculating the interaction index between a drug and a food matrix make sense?

Dear all, I'm conducting the revisions of some of my manuscript, with is the impact of the combination of Aspirin and a fruit juice in colorectal cancer cells. Two of the reviewers have asked to...

16 February 2021 3,141 3 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Why is there cloudy white solution in flavonoid test tube?

I do flavonoid test for biscuit incorporated with muntingia fruit powder. Sample extracted with metanol (1:10 ,w/v), macerated 24h, sentrifuged and filtered. Supernatan used for analysis. I have...

08 February 2021 6,779 4 View

Patients with chronic kidney disease could get COVID-19 vaccine?

04 February 2021 278 4 View

Does the UV radiation affect on fruits ?

I want to examine a kind of fruit's extract's antimicrobial effects. At first I want to put it under the UV radiation to prevent the mold or any kind of fungi's and bacteria's growth. The...

24 January 2021 8,350 20 View

How can you accurately induce calcium overload and measure membrane potential in cultured Tg2576 neuronal cells using whole cell patch clamp method?

Hi there, I'm currently working on a project where i need to design an experiment but as i have little to no experience with this level of experimental design and no face to face help i am really...

22 January 2021 6,728 3 View

Where can I get a Music therapy database for Alzheimer's Disease?

I am working on the effect of music therapy on persons with Alzheimer's Disease. I require EEG signals of healthy individuals and people with Alzheimer's Disease/Dementia that have undergone music...

22 January 2021 8,613 5 View

What is the best nanoparticles that can be easily prepared & can be used in silencing the BACE1 gene for prevention of Alzheimer disease?

I want to do a research about the nanotechnology and management of Alzheimer. I found a lot of ideas about nanoparticles that already studied in that field but I do not know which of them is the...

20 January 2021 1,052 3 View