What is the reason for the lack of DNA bands in gel electrophoresis?

I am working on self-incompatibility by using PCR on Prunus, but I have not yet been able to detect the DNA bands in gel electrophoresis (figure below). I replaced the master mix and the primers...

31 December 2019 430 3 View

What is the reason for decreasing the score in Researchgate?

What is the reason for decreasing the score in RG? For example, despite the fact that I regularly add research items, my citations are raised and I ask questions or answer questions, but each...

11 December 2018 5,953 8 View

What is the cause of this apple fruit disorder?

What is the cause of this color change in apple fruit?

07 August 2018 5,617 1 View

How can scientific findings from research and student theses be commercialized?

Many M.Sc and Ph.D student theses are being conducted annually in the field of agriculture and horticulture, but nobody uses their scientific findings. How can theses be applied and commercialized?

05 June 2018 3,608 4 View

How to collaborate with researchers from other countries?

Despite the problems that exist in scientific relations between researchers in developing countries, how do you think researchers collaborate together? For example, I am very interested in...

05 June 2018 7,993 6 View

What is the cause of browning of apple fruit skin in storage?

The apple fruits bought from the market after a few days in the refrigerator changed their skin color from red to almost brown, while the fruit was healthy and no signs of a pathogen were seen,...

03 April 2018 8,425 10 View

Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference?

Dear colleagues Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference, and how do nutrition, light and growth regulators affect it?

01 February 2018 1,616 0 View

How large is the genome size of the Lepidium draba?

I got the the genome size of Lepidium draba using flow cytometry technique, but since I do not know about the size of its genome (1C or in meiosis), I can not determine the ploidy level. How large...

31 December 2017 4,668 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Oil red O protocol for flow cytometry?

18 February 2021 4,214 3 View