Hello
Does anyone have experience on karyotype, banding or flow cytometry of Lepidium species?
I am working on self-incompatibility by using PCR on Prunus, but I have not yet been able to detect the DNA bands in gel electrophoresis (figure below). I replaced the master mix and the primers...
31 December 2019 674 3 View
What is the reason for decreasing the score in RG? For example, despite the fact that I regularly add research items, my citations are raised and I ask questions or answer questions, but each...
11 December 2018 6,200 8 View
What is the cause of this color change in apple fruit?
07 August 2018 5,964 1 View
Despite the problems that exist in scientific relations between researchers in developing countries, how do you think researchers collaborate together? For example, I am very interested in...
05 June 2018 8,191 6 View
Many M.Sc and Ph.D student theses are being conducted annually in the field of agriculture and horticulture, but nobody uses their scientific findings. How can theses be applied and commercialized?
05 June 2018 3,763 4 View
The apple fruits bought from the market after a few days in the refrigerator changed their skin color from red to almost brown, while the fruit was healthy and no signs of a pathogen were seen,...
03 April 2018 8,610 10 View
Dear colleagues Can someone explain to me the mechanism and process of flowering in plants, or introduce a reference, and how do nutrition, light and growth regulators affect it?
01 February 2018 1,803 0 View
I got the the genome size of Lepidium draba using flow cytometry technique, but since I do not know about the size of its genome (1C or in meiosis), I can not determine the ploidy level. How large...
31 December 2017 4,849 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.
25 July 2024 6,505 2 View
Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...
25 July 2024 2,309 5 View
Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...
25 July 2024 9,058 4 View
Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...
22 July 2024 2,257 0 View
I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.
20 July 2024 6,354 0 View