How one can get copyrights for previously published diagrams?

Usually review articles contain two or more diagrams from previously published research paper. What are the procedures to get copy rights from a journal to reproduce one or two diagrams in a...

25 January 2020 8,121 3 View

How to use fatigue tool for composite in a Ansys workbench?

Currently doing final year project in a leaf spring fatigue test in Ansys workbench 16.0. we have done fatigue test for steel, but error came for composite while testing we don't know how to...

07 March 2019 3,100 2 View

What is the procedure to calculate biomass efficiency?

I have carried out pyrolysis of biomass. Now I am in need of calculating the biomass efficiency (in terms of mass and energy conversion). Kindly help me with a formula.

21 July 2018 457 4 View

Does anyone have experienced the white band pattern in western blot technique for phosphorylated form of AKT protein expression?

03 March 2017 1,441 4 View

How to do Two-way ANOVA in SPSS for four groups and comparing between two different time points within the groups and between the groups?

Hello to everyone I am working in the field of stress physiology, I am comparing different parameters between Male animals Vs Female animals and within Male and female animals, each group contains...

12 October 2016 4,924 2 View

How an atom probe Tomography is advanced from conventional TEM and SEM?

07 October 2016 6,375 5 View

How to select the appropriate statistical method for Questionnaire based survey study?

28 June 2016 9,779 3 View

Can someone provide clarification about the relation between capacitance and zeta potential?

I am working on graphene nanocomposite electrochemistry. I want know about relation between capacitance and zeta potential?

22 February 2015 3,097 4 View

Can someone help with availability of p53 antibody for western blotting providing dealers in India ?

can someone help with availability of p53 antibody for western blotting providing dealers in India ?

30 December 2014 5,036 3 View

What is the maximum ramp rate for power reversal in VSC-HVDC systems?

29 November 2014 597 5 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

I am interested in medicinal chemistry field, which books are highly recommended to learn from?

I am at the beginner level in drug design and drug discovery, and I am interested to learn more about this field. Which books are highly recommended to start from and gives me all essential...

02 March 2021 8,437 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View