I need used 2 % suspension of rabbit erytrocytes for hemagglutination assay. How I can storage erytrocytes long time (2-4 month)?
The following information may be useful for your research
The addition of adenosine to stored blood has been shown to produce a repletion of cellular organic phosphates and an increased ability of the cells to oxidize glucose aerobically (2). Further data will be presented in this paper on the reversal
of other storage changes and in particular the improved maintenance of the post-transfusion\ viability of the erythrocyte.
Rabbit blood, which had been collected in ACD and stored at 4° for three weeks, was divided into two aliquots. Adenosine (2,000,uM per 100 ml. RBC) was added to the first aliquot and saline to the other which served as a control.
Ref:ERYTHROCYTE PRESERVATION. V. RELATIONSHIP BETWEEN
CHEMICAL CHANGES AND VIABILITY OF STORED BLOOD
TREATED WITH ADENOSINE 1
By BEVERLY WESCOTT GABRIO, D. M. DONOHUE, AND C. A. FINCH
www.jci.org/articles/view/103202/files/pdf
Storage duration of 2-4 month is problematic, specifically for RBC from rabbit (cells with short live time). You can use standard storage solution (SAGM, A3 et.c.) .
Additional information:
http://dm5migu4zj3pb.cloudfront.net/manuscripts/103000/103202/JCI55103202.pdf
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