Too low protein concentration for Western Blot - any advice for polyacrylamide gel method?

Currently have punched mouse brain samples of different regions that are of too low protein concentration for Western Blot. Has anyone used the polyacrylamide gel electrophoresis method to detect...

09 January 2021 3,955 3 View

Western Blot: any reasonable alternatives or troubleshooting advice for punched samples?

Hi, I currently have punched mouse brain tissue separated by brain region (using the cryostat) and the initial plan was to do Western Blot analysis on the punched tissue. We already have the...

01 January 2021 8,884 1 View

Does anyone have experience or suggestions on the polyacrylamide gel method of protein purification and measurement for punched samples?

Hello, I currently have sliced and punched mouse brain tissue using the cryostat and I wish to analyze it for the concentrations of different dopamine receptors. We initially desired to use...

01 January 2021 9,455 2 View

How to insert a line of median into scatter plot in SPSS? Multiple variables possible?

Hi! I am using SPSS chart builder to do grouped scatter plots. In the first scatter, I have two groups on the x-axis. I want to add a line of median to each group for better comparison. How can I...

17 November 2020 8,693 3 View

Economic evaluation in mental health services / DataBASE to use?

Hi! I am conducting a master thesis in the field of economic evaluation and mental health services as systematic literature review. Now I consider what databases to use. I have been thinking to...

09 September 2020 2,010 3 View

Can I use in a meta analysis case reports and rcts? And what calculation Do you raccomend( proportion, relative risk....)?

21 August 2020 5,190 3 View

Where can I find credible statistics for social media platforms?

I wonder where I can find credible statistics about patterns of using social networking sites that can be used as a reference in an academic thesis, preferably downloadable. Thanks

08 May 2020 4,932 5 View

Where can I find research papers or data concerning Demographic and Socioeconomic Characteristics of Immigrants and Refugees ?

Hello Everyone, I do hope that all of you are doing well and staying safe amid the COVID-19. I'm trying to find research papers or data that gives a global view about the difference in the...

03 April 2020 4,157 1 View

Why can't I see collagen in SDS-PAGE?

I try to resolve collagen on SDS-PAGE. My goal is to resolve the collagen extracted from skin scaffolds. I use ThermoFisher Bolt mini gels. I can see the protein ladder migrating and also I can...

15 March 2020 9,906 8 View

Your opinion on pulsed field electrophoresis instruments?

Hello, I am interested in running an experiment requiring pulsed field electrophoresis. The primary samples will be budding yeasts chromosomes and minor set of samples with chromosome sizes up...

30 October 2019 7,068 2 View

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02 March 2021 3,060 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View