Freezing mammalian cells in Cell freezing container: can I use this in -150C?

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Andrew Paul McKenzie Pegman

Any temperature is fine as long as you perform the freezing slowly :)

Hanna Dreja

Thanks Andrew Paul McKenzie Pegman. All the commercial freezing containers state -80C. But perhaps I could place this in an outer container.

Yes, minus 80 C is best, but it doesn't matter how low, as long as it is gradual :)