Hi I want to purified a protein for BL21 by sonication. i need the new protocol about that thank you
06 July 2018 7,516 1 View
i want to identify microorganisms involved in organic phosphate and so characterize and isolate genes of organic phosphate catalysis ,then produce one of this enzymes . can u help me? thanks every...
31 December 2015 2,436 0 View
I'm trying to search and find CDS and complete sequence of an enzyme . I have accession number of this enzyme ,the NCBI give me partial sequence so I want to find complete sequence . can you help...
09 October 2015 6,900 2 View
i have some Chickpea samples to extract RNA but i don't have good protocol so i need to good protocol .can you send me protocol if..... i will thanks so much
08 September 2015 1,696 0 View
i means of feature selection is the feature selection in microarrays data and application of Hybrids filter and wrapper Methods for feture selection. and i want the Code Matlab for this issues....
02 March 2015 1,058 2 View
I need this for my thesis
02 March 2015 8,128 4 View
i have some explants in shoot initiation medium ,so i can't take root from them. what is the best method to indution of shoot elongation. can i use low tempreture to induction?
01 January 1970 7,734 3 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View
I use 0.5% sarkosyl to solubilise recombinant proteins contained in Inclusion bodies of E. coli. sarkosyl very effeciently solubilizes proteins from Inclusion bodies and I am trying to use...
25 July 2024 5,001 2 View
"The Correlation between Nutrition and Transport Mechanism under Abiotic Stress in Plants: A Comprehensive Review" by Muhammad Saleem, Jianhua Zhang, Muhammad Qasim, Rashid Iqbal, and Li Song,...
23 July 2024 9,109 0 View
Good morning, I am trying to produce a recombinant protein. Unfortunately I one of the two restriction enzymes that I use do not cut at the end of the MCS. After the digestion 15 nucleotides of...
17 July 2024 2,787 4 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
29 June 2024 9,688 1 View
My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.
24 June 2024 5,044 1 View
Hi everyone, I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel. I've been adding all reagents minus template, to make a master mix...
24 June 2024 1,171 3 View
Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...
24 June 2024 8,622 6 View