Can anyone give the protein purification protocol by soniaction?

Hi I want to purified a protein for BL21 by sonication. i need the new protocol about that thank you

06 July 2018 7,443 1 View

How i can restrict a part of a CDS ?

10 November 2015 7,917 0 View

I have partial sequence of a gene for an enzyme .how can i find CDS and entire sequence of this enzyme ?

I'm trying to search and find CDS and complete sequence of an enzyme . I have accession number of this enzyme ,the NCBI give me partial sequence so I want to find complete sequence . can you help...

09 October 2015 6,848 2 View

I want to extract RNA by biozol , can you help me?

08 September 2015 1,631 0 View

Do you know the feature selection Codes for Hybrid approach filter_wrapper Algorithms?

i means of feature selection is the feature selection in microarrays data and application of Hybrids filter and wrapper Methods for feture selection. and i want the Code Matlab for this issues....

02 March 2015 995 2 View

Do you know about Algorithm matlab codes for feature selection?

I need this for my thesis

02 March 2015 8,074 4 View

What can i do to elongation of shoots for canola in media culture ?

01 January 1970 7,673 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

How does the gut microbiome affect the fibrotic process within the liver?

and Can the gut microbe be used for the treatment or diagnosis of liver fibrosis?

02 March 2021 557 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View