Hi everyone,
I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel.
I've been adding all reagents minus template, to make a master mix of the restrictions reagents.
I am wondering if that would be the cause of the unsuccessful reaction.
And that I should add the reagent individually/separately instead for each sample and not do a mastermix.
Greatly appreciate any advice from anyone who has experience in running successful digestion reaction.