Hi everyone,

I am currently trouble shooting my restriction digestion, so far it hasnt given me clear bands when I ran gel.

I've been adding all reagents minus template, to make a master mix of the restrictions reagents.

I am wondering if that would be the cause of the unsuccessful reaction.

And that I should add the reagent individually/separately instead for each sample and not do a mastermix.

Greatly appreciate any advice from anyone who has experience in running successful digestion reaction.

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