i have some Chickpea samples to extract RNA but i don't have good protocol so i need to good protocol .can you send me protocol if..... i will thanks so much
Hi I want to purified a protein for BL21 by sonication. i need the new protocol about that thank you
06 July 2018 7,516 1 View
i want to identify microorganisms involved in organic phosphate and so characterize and isolate genes of organic phosphate catalysis ,then produce one of this enzymes . can u help me? thanks every...
31 December 2015 2,436 0 View
i have CDS of a protein and I want to restrict part of it what should I do? thanks every body
10 November 2015 7,981 0 View
I'm trying to search and find CDS and complete sequence of an enzyme . I have accession number of this enzyme ,the NCBI give me partial sequence so I want to find complete sequence . can you help...
09 October 2015 6,900 2 View
i means of feature selection is the feature selection in microarrays data and application of Hybrids filter and wrapper Methods for feture selection. and i want the Code Matlab for this issues....
02 March 2015 1,058 2 View
I need this for my thesis
02 March 2015 8,128 4 View
i have some explants in shoot initiation medium ,so i can't take root from them. what is the best method to indution of shoot elongation. can i use low tempreture to induction?
01 January 1970 7,734 3 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?
09 August 2024 7,718 0 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Palmitic acid presence in aqueous fraction
05 August 2024 8,624 4 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View