What is the ratio between sample and solvent in marine animal carotenoids and other pigment extraction?

For freeze dry sample, (sample (g)/solvent (acetone (ml)?

08 September 2014 211 3 View

I nee information about zooxanthellae degradation by using sea anemone tissue homogenized for zooxanthellae isolation.

i want to compare isolation method and histology method (prepare slid and counting zooxanthelea in image) method to determine one of them is better for density consideration.

07 August 2014 4,308 0 View

( Can anyone suggest any articles on histomorphological changes during the sea anemone reproduction? )

when (month or season? ) sea anemone (stichodactyla) has sexual reproduction?

05 June 2014 6,770 0 View

How can I perform zooxanthellae numeration and count the density of zooxanthellae with sea anemone histology?

With imagej or another software?

05 June 2014 727 4 View

For injection of Carotenoids sample to HPLC what ratio and how much solvent (acetonitrile and methanol) is needed?

What's the ratio? How much methanol and acetonitrile do I need for prepared solution?

05 June 2014 9,847 4 View

How can I separate chlorophyll and carotenoids in pigment extraction with acetone?

i prepared sea anemon pigment extraction that i should separate chlorophyll to achieve just carotenoids, how can i do it?

05 June 2014 7,461 3 View

Can anyone provide me with a carotenoid HPLC analysis protocol?

What solvent should I use for a carotenoid injection into an HPLC system (after drying sample). Is HPLC solvent for animal carotenoids different from other organisms, for example vegetable or...

05 June 2014 277 4 View

How can I compare seasonal changes in zooxanthellae density with sea anemone histology?

I am looking for a statistical analysis of this problem.

05 June 2014 9,628 0 View

Can anyone recommend a book or paper containing high-quality histological images and methods of sea anemone?

About sea anemone, sea carpet (stichodactyla) or invertebrate.

02 March 2014 2,128 5 View

How can you prepare total carotenoids for HPLC method?

Thanks.

01 February 2014 4,351 3 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View