What is the ratio between sample and solvent in marine animal carotenoids and other pigment extraction?

For freeze dry sample, (sample (g)/solvent (acetone (ml)?

08 September 2014 211 3 View

I nee information about zooxanthellae degradation by using sea anemone tissue homogenized for zooxanthellae isolation.

i want to compare isolation method and histology method (prepare slid and counting zooxanthelea in image) method to determine one of them is better for density consideration.

07 August 2014 4,308 0 View

how i can add carotenpoids to cell culture (what solvent i need to solve carotenoids) ?

what solvent? i should complete dry carotenoids extract then solve carotenoids in?

05 June 2014 9,477 0 View

( Can anyone suggest any articles on histomorphological changes during the sea anemone reproduction? )

when (month or season? ) sea anemone (stichodactyla) has sexual reproduction?

05 June 2014 6,770 0 View

How can I perform zooxanthellae numeration and count the density of zooxanthellae with sea anemone histology?

With imagej or another software?

05 June 2014 727 4 View

For injection of Carotenoids sample to HPLC what ratio and how much solvent (acetonitrile and methanol) is needed?

What's the ratio? How much methanol and acetonitrile do I need for prepared solution?

05 June 2014 9,847 4 View

How can I separate chlorophyll and carotenoids in pigment extraction with acetone?

i prepared sea anemon pigment extraction that i should separate chlorophyll to achieve just carotenoids, how can i do it?

05 June 2014 7,461 3 View

Can anyone provide me with a carotenoid HPLC analysis protocol?

What solvent should I use for a carotenoid injection into an HPLC system (after drying sample). Is HPLC solvent for animal carotenoids different from other organisms, for example vegetable or...

05 June 2014 277 4 View

How can I compare seasonal changes in zooxanthellae density with sea anemone histology?

I am looking for a statistical analysis of this problem.

05 June 2014 9,628 0 View

Can anyone recommend a book or paper containing high-quality histological images and methods of sea anemone?

About sea anemone, sea carpet (stichodactyla) or invertebrate.

02 March 2014 2,128 5 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

Why almost every element would be metallized in sufficiently high pressure and low temperature?

(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...

02 March 2021 3,309 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

I have designed reflectarray element and feeding it though Horn Antenna on HFSS. After full wave simulation, I am not getting desired results?

I have designed reflectarray element to generate OAM beams at 5.8GHz and feeding it though Horn Antenna on HFSS. After full wave simulation, i am not getting desired results i.e Radiation pattern...

01 March 2021 9,701 5 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

Microstrip antenna design tutorial of HFSS 3D layout module ?

I have designed a 24 GHz rectangular antenna on HFSS 3D model and got desired results while desing the same antenna with same dimensions I am getting resonance frequency at 27 GHz I am...

28 February 2021 4,426 2 View