What is the ratio between sample and solvent in marine animal carotenoids and other pigment extraction?

For freeze dry sample, (sample (g)/solvent (acetone (ml)?

08 September 2014 211 3 View

I nee information about zooxanthellae degradation by using sea anemone tissue homogenized for zooxanthellae isolation.

i want to compare isolation method and histology method (prepare slid and counting zooxanthelea in image) method to determine one of them is better for density consideration.

07 August 2014 4,308 0 View

how i can add carotenpoids to cell culture (what solvent i need to solve carotenoids) ?

what solvent? i should complete dry carotenoids extract then solve carotenoids in?

05 June 2014 9,477 0 View

( Can anyone suggest any articles on histomorphological changes during the sea anemone reproduction? )

when (month or season? ) sea anemone (stichodactyla) has sexual reproduction?

05 June 2014 6,770 0 View

How can I perform zooxanthellae numeration and count the density of zooxanthellae with sea anemone histology?

With imagej or another software?

05 June 2014 727 4 View

For injection of Carotenoids sample to HPLC what ratio and how much solvent (acetonitrile and methanol) is needed?

What's the ratio? How much methanol and acetonitrile do I need for prepared solution?

05 June 2014 9,847 4 View

How can I separate chlorophyll and carotenoids in pigment extraction with acetone?

i prepared sea anemon pigment extraction that i should separate chlorophyll to achieve just carotenoids, how can i do it?

05 June 2014 7,461 3 View

Can anyone provide me with a carotenoid HPLC analysis protocol?

What solvent should I use for a carotenoid injection into an HPLC system (after drying sample). Is HPLC solvent for animal carotenoids different from other organisms, for example vegetable or...

05 June 2014 277 4 View

How can I compare seasonal changes in zooxanthellae density with sea anemone histology?

I am looking for a statistical analysis of this problem.

05 June 2014 9,628 0 View

How can you prepare total carotenoids for HPLC method?

Thanks.

01 February 2014 4,351 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

Reagents for histology slides?

Dear all, I am planning to study histology and want to do that at home. I have only stained a blood smear with Giemsa but now want to try a wider range of animal tissues. What reagents will I...

24 February 2021 5,430 3 View

Any recommendations for methods to analyze elephant space-use (using GPS) and correlating this with changes in NDVI?

For my Masters I am looking into elephant space-use and its effect on vegetation change. I have elephant GPS collar data and was going to use Google Earth Engine to obtain NDVI values. I would...

16 February 2021 7,097 3 View

Does anyone have any experience with the Motic EasyScan Pro Slide Scanner?

I am interested in purchasing a slide scanner for histology and was presented with the Motic EasyScan Pro but I have never heard of the company, nor know of anyone who has used the system. Can...

11 February 2021 2,416 2 View

Having trouble mounting the sections on the slides for IHC staining, what solution should I use?

Hello everyone, I just started learning IHC staining and I have some frozen sections to practice with in a 96-well plate. I'm having trouble mounting the sections on the slide, a colleague told me...

08 February 2021 6,880 3 View

How to fix and protect blood clots for histology ?

Hi, I encounter some problems, I need to my research to fix and cryoprotec some blood clot for histology analyses. Mys current protocol is the following : 48h of para formaldehyde 4% and 7 days of...

07 February 2021 3,756 3 View

Which isomer of xylene do you use in hystology ?

Which isomer of xylene do you use for the preparation of histological tissue sections - meta para or ortho?

02 February 2021 833 4 View

Is it possible estimating environmental conditions with a Bayesian approach?

In ecology specific species can be used to get an indication/estimation of the environmental conditions. A simplistic example, lets assume I find a pike (Esox lucius). We know this species does...

01 February 2021 10,034 4 View

How do you annotate figures to show significance?

30 January 2021 1,008 3 View