Please if we want to check many related species in the same eDNA sample we should use more primers or more samples or sequence all genome extracted from samples
I understand that You have samples from environment which contain mixture of species and You want to identify that species in samples , correct ?
Depending on what taxonomic level you are looking at and what range of species you are looking at will determine what type of primers you need to use.
Ie Folmer Primers can amplify a 700bp region of COI in 11 invertebrate phyla
I am so grateful for your reply dear colleagues.
Actually I want to checking the species (belong to same family) in an environment. All of those species barcode by one primer so how we can determine the species composition by using the same primer.
Dear Leah Clarke : Is this technique is efficient to investigate species composition even if they related with the same genus or family. Please reply
Thank you for all of you Dr. Combik, Dr. Clarke and Dr. Laldin.
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