How to extract protein after trizol RNA and back extraction of genomic DNA?

I have limited amount of samples, so I am trying to get RNA, DNA and protein from the same samples. I used Trizol reagent for RNA isolation, then use back extraction buffer for genomic DNA...

13 January 2021 1,566 2 View

Where can I find solar irradiation data with high resolution?

I am trying to generate a 1 day solar irradiation profile. Any suggested high resolution(possibly in minutes) source for the same?

06 December 2020 5,976 18 View

How to implement only a Gate with activation sigmoid or tanh in keras?

I want to implement only a Gate similar to forget gate or output gate of LSTM cell. Mind it...!!! I am not implementing the entire LSTM cell rather I have certain input and previous output and I...

22 September 2020 8,236 3 View

Could someone help me interpret the following PMF curve, especially the physical reason behind the multiple potential wells?

I am simulating the pulling of two nanoparticles in a polymer. I get the attached PMF curve, showing a secondary (and much deeper) potential well. I was wondering if there is a physical...

19 September 2020 7,504 5 View

How to determine relationship among the independent variables to get lowest MSE (Mean Squared Error) in prediction?

I have a dependent variable A, which can be perceived from B, C and D. So to say A = function(B,C,D). B, C, D results in A that is known to us. However, I don't know whether B,C and D follow a...

28 June 2020 6,332 6 View

IS it Necessary for Primers in Taq-Man PCR to be HPLC Purified?

I want to set up a taq man reaction. I want to know that in taq man reaction I have to order HPLC purified Primers or ordinary primers are adequate for me. If you have experience in taq man,...

23 June 2020 5,511 3 View

No title

We're looking at storing serum and plasma samples from a clinical trial. I've looked at various papers, but the only place where I found some hazy answer was in the supplementary. But I'm hoping,...

04 March 2020 8,858 3 View

What are the various possible applications of Machine Learning in the problems of FLuid Mechanics where it is necessary? ?

I have seen researchers are more inclined towards machine learning application nowadays . My interest is to know the various application of fluid mechanics problem where machine learning can bring...

27 February 2020 8,809 3 View

How to calculate the required motor HP to run a machine?

Hello every one, Can some body help me in calculating the required HP for motor to run the machine which includes transmission system with 2 Nos of shaft and cutting blades.

28 December 2019 9,788 3 View

Is there any benchmark dataset for distributed camera networks?

I am particularly looking for dataset containing cameras capturing videos in a distributed setup. The videos, captured by multiple cameras have to be correlated. Is there any benchmark dataset on...

22 November 2019 9,567 3 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Cloudy/high background western blot - can anyone help me?

Hi everyone! I try to detect VDR protein (~48 kDa) using Western Blot. After SDS-PAGE using 10% MP TGX Stain-Free Gel from BioRad I transferred the protein onto an metOH activated PVDF membrane...

23 February 2021 6,266 3 View

How to prepare electrolyte of NaClO4 salt for Na-ion battery? Any problem with this salt?

Hello, I am trying to make anode for Na-ion batteries. I have no experience with preparation of the electrolyte for any batteries. NaClO4 salt are available and I am planning to use EC:PC as...

21 February 2021 8,132 1 View

Protocol for membrane extraction E. coli?

Hello! I am interested in determining whether a poly-unsaturated fatty acid I have engineered E. coli to produce is being incorporated into the membrane of the cells or if it is simply a free...

21 February 2021 7,447 2 View

Do you know this error? The experiment was stopped by a potential overload on Vs (auxiliary electrode)?

Hello everyone Do you have any information about this error 'The experiment was stopped by a potential overload on Vs (auxiliary electrode)' in Origamaster software? I tried everything possible,...

19 February 2021 3,152 7 View

Is there a paper that shows the effect of the thermal conductivity of solid electrolytes on lithium secondary batteries?

I want to know the effect of thermal conductivity characteristics of solid electrolytes on the all-solid battery, but I don't know which paper to refer to. Is there a paper on thermal behavior?

15 February 2021 8,806 2 View

How do you prepare a porous crosslinked Polyvinyl alcohol and Glutaraldehyde membrane or film?

I am trying to prepare a Polyvinyl alcohol membrane for carbondioxide adsorption

08 February 2021 7,493 3 View

Background and spots in Western Blot membrane?

Do you know why my membrane looks like this? I did this western for Anti-hnRNP D/AUF1 antibody ab50692, as primary antibody in 1:1000 overnight at 4c and Anti-rabbit IgG HRP linked antibody...

07 February 2021 5,591 4 View