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Questions related from Jawwad Ahmad
If I have only two options in front of me to select either Gasteiger or the AM1-BCC, so based on which parameters or rules I can select the most appropriate charge scheme during minimization step...
19 July 2020 8,938 1 View
Hi everyone, if I do not use spike-in RNA isoform controls for a customized RNA sequencing experiment (not the whole transcriptome, just based on few specific genes we are interesting), so is...
09 February 2020 7,306 2 View
Dear All, 1 ug of permissible quality FFPE RNA is transcribed to cDNA through SSIV VILO RT kit. I have Qubit v.4 device, which can measure cDNA to a good extent. The cDNA ranges from 25-100 ng /...
12 November 2019 2,139 1 View
It is a known fact now that interactions between bacterial (normal flora) and colon cancer cells influence the transcription profile of host cells or enterocytes. So, how can I avoid or minimize...
12 October 2018 9,587 1 View
If I am interested in a customized mRNA-only Sequencing (RNA-Seq) based on few transcripts (not the whole transcriptome), and after having satisfied & permissible RIN values for my extracted...
22 September 2018 1,904 3 View
Dear all, in a PCR (conventional & realtime both), if we achieve our desired product (GOI) and along with, there is also no primer dimer (PD) formation seen on the gel and melt curve, does...
03 November 2017 10,040 3 View
Any explanative thoughts or information? Will the 2nd or 3rd melt of products attribute to some improvement for their detection?
21 October 2017 8,670 3 View
A low concentration of a primer than the other one in high concentration or even in volume often exhibits good melt curve appearances. What could be the reasons behind this? Is this dependent on...
28 November 2016 7,782 3 View
So if any gene which is not in a single copy form, can we use it?
21 November 2016 6,606 3 View
And how to recognize such amplifications on the basis of Tm in the melt curve stage? As it is a known fact that retropseudo genes have no introns and they can be amplified at cDNA level along...
07 November 2016 7,636 2 View