How do you prepare a protein to coat a reverse ELISA plate?

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Jai Ghosh

I would suggest that you use normal sodium chloride solution or even a low concentration of phosphate buffer to coat the wells for reverse ELISA

Pongpawan Sethanant

Normally I just use protein in bicarbonate buffer (pH 9.6) for coating the proteins on the plate (Nunc-Maxisorp). Methanol or other denaturants might not work well with every assay in the case that your antibody recognizes a conformational epitope.

Wolfgang Schechinger

Is the MeOH precipitation part of a purification procedure? Some proteins are soluble in methanol.