I want to quantify 2-HG metabolite in the cultured cells and after drug treatment. can the cells be frozen if not performing the lysis on the same day?
You need to freeze them immediately using liquid nitrogen in order to stop the metabolism of the cell. After this you can keep the cells until the analysis day.
Yes, cells need to be frozen immediately, if it's not possible to proceed on the same day. This freezing is the first or recommend step during the metabolite extraction.