05 October 2017 4 10K Report

I would like to run radiolabelled cDNA on an alkaline agarose gel, dry it so it fits in the cassette and then expose the xray film.

Has anyone tried drying agarose gels without a gel dryer while still maintaining the integrity of the run samples?

There's a paper from 1988 that says "fragments of DNA of 1000 bp or less in length are readily lost during drying" and that it helps to fix the DNA with CTAB or use a membrane to avoid losing the material. But they use a gel dryer.

Alternatively, does anyone have experience drying agarose gels using gel dryers intended for acrylamide gels (Biorad 583)?

I could try just running a ladder first and see for myself but would really appreciate some tips and tricks.

Thanks in advance!

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