When drawing a phylogenetic tree with somatic mutations(SNPs), what is the role of tree simulation process?

Hello, I am working on drawing a phylogenetic tree with samples sharing some somatic mutations. The basic process of my work is similar to this article. Population dynamics of normal human blood...

14 October 2020 8,078 4 View

In grignard reaction, how can I attach 'Mg' to only one side of dibromoalkane?

As you can see in the image file, I want to make Br-C12-dithiocarboxylic acid form. However, I don't know how to control the amounts of reactant. I tried 'Mg:alkane=1:1 equiv' but many byproducts...

18 September 2020 3,970 4 View

I would like to mix FBS and horse serum (1:1) when culturing cells, do you think it would change sequencing results?

Hello, I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end) Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1) I have done...

10 September 2020 8,137 3 View

Is there any way I can do spheroid culture with fibroblasts (not cancer cell)?

I have done spheroid culture with various cancer cell lines The spheroid was formed from single cells. The method was like the added picture (not curved bottom well, matrigel, etc) The question...

30 March 2020 9,817 3 View

Why use dol dme electrolyte in lithium sulfur batteries mostly?

In lithium metal battery with transition metal cathode, they use lipf6 salt and different electrolyte. However, in lithium sulfur batteries, almost studies were conducted under dol dme electrolyte...

30 March 2020 9,603 4 View

Some of SH-SY5Y cells detach during media exchange in 96well How can I deal with?

Hi all, I seeded 1 X 10^4 of SH-SY5Y on 96 well plate and grew them overnight. For drug experiment, I removed all media using pipette and treated media mixed with drug. However, I found that some...

23 March 2020 8,924 4 View

Would you let me know how to extract Polysaccharides?

I'm planning to extract Polysaccharide from Codium fragile, which is green algae. I used to use hot water for extraction for 3 hours and then use Ethanol to get them. I tried this way, but It...

17 September 2019 4,558 5 View

About photonic crystal, Is there any conditions that minimum number of polymer layers of particle which can color?

I understand that incresing number of layers causes high reflectance..but I don't know the minimum condition that particle can be used as photonic crystal..

08 August 2019 1,413 1 View

Vimentin Western Blot: No consistency of the change in vimentin expression level of A549 cell line. What can I do?

I am working on Epithelial to Mesenchymal Transition (EMT) in A549 cell line by treating positive inducers such as EGF to up-regulate the mesenchymal markers like vimentin. I'm doing western blot...

22 May 2019 3,053 1 View

Can I use this live/dead cell assay kit after I fixed the samples?

https://www.thermofisher.com/order/catalog/product/L3224 This is the live/dead cell assay kit I will use for my experiment. But I have to fix samples (cancer cells) before using this kit, maybe...

29 April 2019 3,481 3 View

How to match the XRD of a compound for which there is no JCPDS file ? Is there any other characterization to claim the synthesis of desired compound?

I have synthesized LiMgF3 using the starting precursors LiF and MgF2 via solid state diffusion method. Now I am looking for some characterization to claim the desired product, as there is no...

09 February 2021 921 3 View

Asked a question in SnO2 ?

08 December 2020 6,957 2 View

Why in some papers people have shown one jcpds card no. for two different metals?

most people have shown I their research articles that the intensity peaks of different metals like ag, au and even pd have their peaks on the same degrees i.e. nearby 38.2, 44.2,64.6, 77.5 degrees...

05 August 2020 7,751 4 View

Can anyone provide me all the reference JCPDS cards for ag, au and pd?

I need these reference cards to match them with my data. if you can help then kindly provide the pdf files or even images for each card. I do have one reference card for each but it lefts me the...

05 August 2020 6,147 4 View

Please , I need (XRD) data sheet standard (JCPDS manual). for ( hydroxyapatite, multi-walled carbon nanotubes, High-density polyethylene ) materials?

XRD data sheet standard JCPDS manual

01 February 2020 7,225 3 View

I need JCPDS file for Ag, Au, Pt and Pd Nanoparticles (NPs were synthesized using biomaterials). Can you help me please?

11 December 2019 793 4 View

Where can I find an online resource to get XRD JCPDS files?

We are looking for to find xray card for my organic dyes to specified Miller indices present in XRD powder e.g (c39H44N606)(C18H26N9O6) Best regards and thank you very much for your help and...

12 November 2019 8,699 2 View

What are the possible reasons of XRD peak deviation, when compared to standard data?

I have XRD data of synthesized material in powder form and when I compare it with standard JCPDS data, there is shift in peak positions which is not constant, ranging from 0.2 to 0.6 degree from...

10 November 2019 1,387 4 View

Can somebody share NiO JCPDS file?

Can somebody share NiO JCPDS file

09 October 2019 2,206 3 View

What the best program for data analysis of XRD?

Jade 6 is the best program for data analysis of XRD, because you can get JCPDS directly from this program.

07 August 2019 4,882 2 View