When drawing a phylogenetic tree with somatic mutations(SNPs), what is the role of tree simulation process?

Hello, I am working on drawing a phylogenetic tree with samples sharing some somatic mutations. The basic process of my work is similar to this article. Population dynamics of normal human blood...

14 October 2020 8,078 4 View

In grignard reaction, how can I attach 'Mg' to only one side of dibromoalkane?

As you can see in the image file, I want to make Br-C12-dithiocarboxylic acid form. However, I don't know how to control the amounts of reactant. I tried 'Mg:alkane=1:1 equiv' but many byproducts...

18 September 2020 3,970 4 View

I would like to mix FBS and horse serum (1:1) when culturing cells, do you think it would change sequencing results?

Hello, I am doing fibroblast, stem cell culture for sequencing. (From primary culture to the end) Since FBS is expensive, there are some protocols that mix FBS and horse serum (1:1) I have done...

10 September 2020 8,137 3 View

Is there any way I can do spheroid culture with fibroblasts (not cancer cell)?

I have done spheroid culture with various cancer cell lines The spheroid was formed from single cells. The method was like the added picture (not curved bottom well, matrigel, etc) The question...

30 March 2020 9,817 3 View

Why use dol dme electrolyte in lithium sulfur batteries mostly?

In lithium metal battery with transition metal cathode, they use lipf6 salt and different electrolyte. However, in lithium sulfur batteries, almost studies were conducted under dol dme electrolyte...

30 March 2020 9,603 4 View

Some of SH-SY5Y cells detach during media exchange in 96well How can I deal with?

Hi all, I seeded 1 X 10^4 of SH-SY5Y on 96 well plate and grew them overnight. For drug experiment, I removed all media using pipette and treated media mixed with drug. However, I found that some...

23 March 2020 8,924 4 View

Would you let me know how to extract Polysaccharides?

I'm planning to extract Polysaccharide from Codium fragile, which is green algae. I used to use hot water for extraction for 3 hours and then use Ethanol to get them. I tried this way, but It...

17 September 2019 4,558 5 View

About photonic crystal, Is there any conditions that minimum number of polymer layers of particle which can color?

I understand that incresing number of layers causes high reflectance..but I don't know the minimum condition that particle can be used as photonic crystal..

08 August 2019 1,413 1 View

Vimentin Western Blot: No consistency of the change in vimentin expression level of A549 cell line. What can I do?

I am working on Epithelial to Mesenchymal Transition (EMT) in A549 cell line by treating positive inducers such as EGF to up-regulate the mesenchymal markers like vimentin. I'm doing western blot...

22 May 2019 3,053 1 View

Can I use this live/dead cell assay kit after I fixed the samples?

https://www.thermofisher.com/order/catalog/product/L3224 This is the live/dead cell assay kit I will use for my experiment. But I have to fix samples (cancer cells) before using this kit, maybe...

29 April 2019 3,481 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

In an eye with Tyndall, does each dot correspond to an individual cell?

When you observe the presence of Tyndall in an inflamed eye: Does each of the small floating dots correspond to an individual cell or to an aggregate of cells? Does a conventional slit lamp have...

27 February 2021 6,326 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View