I need to study the effect of the protein Y mutation on the levels of a protein X. So I over-expressed the protein X under 5 conditions:
(1) not over-expression of protein Y
(2) with over-expression of a wild type protein Y
(3, 4, 5) with over-expression of Y mutants
The over-expression of the Y mutants is not the same due to the mutation. Because of these differences probably I should normalise the X protein level with tubulin but also with the normalised levels of the mutants?
How can I compare the X protein levels to get some information about the effect of Y mutation on X protein level?